| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149790 |
| Chromosome: | chromosome 15 |
| Location: | 384586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636004 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCTACGGCGACGAGTTCTCCGATGACC |
| Internal bar code: | TATGTGTGGTCGTGCGAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 73.1971 |
| LEAP-Seq n confirming: | 1218 |
| LEAP-Seq n nonconfirming: | 446 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTTTGGAAAAGCGGTA |
| Suggested primer 2: | CCTCTTGACTACTCCGACGC |