| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149801 |
| Chromosome: | chromosome 14 |
| Location: | 1872169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620652 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGGTTGTGCCGCCCTCGAGTCCGAAT |
| Internal bar code: | GTTAAACTTGATAGGCGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 96.9492 |
| LEAP-Seq n confirming: | 572 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCTCAAGCGTCAAGGAA |
| Suggested primer 2: | GACTCCTGCTTCGTCCTTTG |