| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149805 |
| Chromosome: | chromosome 16 |
| Location: | 5887744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677300 | GOX16 | Glyoxal oxidase 16; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGGTGTAGGCAGCAAGGCAGCATGCGT |
| Internal bar code: | TCGGGTTGGCTGAACCGAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.9146 |
| LEAP-Seq n confirming: | 2340 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGACCGGAAGGTTTCT |
| Suggested primer 2: | ACATAATCCCCGGAAACCTC |