| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149805 |
| Chromosome: | chromosome_16 |
| Location: | 5887746 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g677300 | GOX16 | Glyoxal or galactose oxidase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAATGCAATGTCCGCTTACTTTCGGCTCC |
| Internal bar code: | CACGGACCATGGTTCCGTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 99.6397 |
| LEAP-Seq n confirming: | 6360 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGACCGGAAGGTTTCT |
| Suggested primer 2: | ACATAATCCCCGGAAACCTC |