| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149835 |
| Chromosome: | chromosome 3 |
| Location: | 2658795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161000 | ADA1 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.4 - Adenosine deaminase / Adenosine aminohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCCCACCACCCCGCGGGACTGCAGG |
| Internal bar code: | GAACGGACTCCCAGCGAGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.8821 |
| LEAP-Seq n confirming: | 847 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTATTGGTCCGTACTGCG |
| Suggested primer 2: | GTACGGCATGACCAAGGAGT |