| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149838 |
| Chromosome: | chromosome 12 |
| Location: | 2440672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507000 | FFT6 | putative fructan fructosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACAGTGCGTGGACATTACTCACGTCAG |
| Internal bar code: | GGACAGAGTGTGTGTCCGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 99.681 |
| LEAP-Seq n confirming: | 1250 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGATGTTGGGTATGGG |
| Suggested primer 2: | TGAAGAGGTTTCGGCTGAGT |