Insertion junction: LMJ.RY0402.149853_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149853
Chromosome:chromosome 7
Location:5603264
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g351900 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCGCCAAACCTGAACGCACACACCCCAA
Internal bar code:CGAAAATACAGTCAGGATGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:99.7719
LEAP-Seq n confirming:875
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTCATTACAGGCCATGC
Suggested primer 2:CATGACATTCGCGAGCTTTA