| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149864 |
| Chromosome: | chromosome 10 |
| Location: | 372952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420500 | (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGCATGAAGCAGTATGGAATTCAGTC |
| Internal bar code: | TCCGCCTAACCCTTCCAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAAGAACGATACCTGC |
| Suggested primer 2: | TTAAGTAATGGTCAGCCGCC |