| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149880 |
| Chromosome: | chromosome 17 |
| Location: | 2000590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711250 | MIP2 | (1 of 2) PTHR19139:SF180 - AQUAPORIN-1; Aquaporin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCTGTGCCTTCTTCACTCTGCAACAAG |
| Internal bar code: | GGACTGGAATTCATTCGACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.5979 |
| LEAP-Seq n confirming: | 743 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGGAGTAGGCAGGAAC |
| Suggested primer 2: | GCATACATTCACGATGCCAC |