| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149883 |
| Chromosome: | chromosome 9 |
| Location: | 811884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401950 | (1 of 8) 2.7.11.12 - cGMP-dependent protein kinase / PKG II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGCCTCCCGCAGTTTGGGGAAGTGGG |
| Internal bar code: | TCTTTGCGCGGCATTTGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.5022 |
| LEAP-Seq n confirming: | 1599 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTACCCTAGCAGACCCC |
| Suggested primer 2: | AGCTTCGGCAAGATCCAGTA |