Insertion junction: LMJ.RY0402.149890_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149890
Chromosome:chromosome 1
Location:7867603
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g060347 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTGGGCGCATGCCCGGCACGCCTGGGC
Internal bar code:ACTGGTCCGCGGAGTTCCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:260
LEAP-Seq percent confirming:99.5025
LEAP-Seq n confirming:1000
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCAGGATAGCGCGTAG
Suggested primer 2:AGTGCCAACGATACTGTCCC