| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149910 |
| Chromosome: | chromosome 10 |
| Location: | 3457196 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGTTGGATGCTGCACAATCATCGTGCG |
| Internal bar code: | GATGACTCAGGTGAGTCTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.4156 |
| LEAP-Seq n confirming: | 14119 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGGCTGTGATACTGTG |
| Suggested primer 2: | CATAGACAGGCGCTGAAACA |