Insertion junction: LMJ.RY0402.149912_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.149912
Chromosome:chromosome 6
Location:3747955
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278153 (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGTAGAAGTAGCAAGAGCGCAAAAGTGC
Internal bar code:TTTTCCGAAAGACGGCTTATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1027
LEAP-Seq percent confirming:99.8039
LEAP-Seq n confirming:509
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGCGTGCGTACGTGTAT
Suggested primer 2:TGTCAAGTTGTCAACCCCAA