| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149948 |
| Chromosome: | chromosome 12 |
| Location: | 3918167 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516050 | (1 of 1) PF00498//PF13920 - FHA domain (FHA) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAATGGAAGGAGATGTTGCGACACCGGC |
| Internal bar code: | ACGCTTTAAGTACAAAAATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 98.8108 |
| LEAP-Seq n confirming: | 3573 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTGTACGTGTTTCGGA |
| Suggested primer 2: | GACACTGCTGGTTGTGCAGT |