| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149958 |
| Chromosome: | chromosome 9 |
| Location: | 6856967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410000 | (1 of 1) PTHR13604//PTHR13604:SF0 - DC12-RELATED // EMBRYONIC STEM CELL-SPECIFIC 5-HYDROXYMETHYLCYTOSINE-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCGGTTTGTTTCGCAGGTGGGGCCTGG |
| Internal bar code: | GGGGAACAATATCGAAGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 97.8056 |
| LEAP-Seq n confirming: | 312 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGCGGACTTGGTAGAAG |
| Suggested primer 2: | ATAGAAGCCGTCCAGAAGCA |