| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149965 |
| Chromosome: | chromosome 12 |
| Location: | 3513418 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513100 | (1 of 1) 3.2.1.165 - Exo-1,4-beta-D-glucosaminidase / GlcNase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCACTACGACATGGCAGCCGCAGCAG |
| Internal bar code: | GGGTGCCACGCTCGGCCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.8974 |
| LEAP-Seq n confirming: | 974 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCAGTGGTTGTTTGGCT |
| Suggested primer 2: | GAGCAGCGCCCAGTACTATC |