Insertion junction: LMJ.RY0402.149983_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.149983
Chromosome:chromosome 5
Location:194122
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g234200 (1 of 1) IPR001374//IPR020683 - R3H domain // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCAGACACCCAGCCAGTCAGTACAGTCT
Internal bar code:CCCCCCAGTAACTTTGCGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:896
LEAP-Seq percent confirming:99.0245
LEAP-Seq n confirming:13907
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAAAAGGGTTGATTGTG
Suggested primer 2:CATCGGTGTCGGACATACTG