Insertion junction: LMJ.RY0402.150012_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.150012
Chromosome:chromosome 5
Location:2206390
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g234654 (1 of 1) IPR002110//IPR003613//IPR013083//IPR020683 - Ankyrin repeat // U box domain // Zinc finger, RING/FYVE/PHD-type // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGATGCTCGCTGACCCTATCATCGTTGC
Internal bar code:GTCAAGGGCCTTACGCCGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:98.8701
LEAP-Seq n confirming:350
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTACTGCTGAAACCAAC
Suggested primer 2:GCGGAACTAGGTGTGGATGT