| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150015 |
| Chromosome: | chromosome 9 |
| Location: | 659483 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403200 | FAP198 | Flagellar Associated Protein 198; (1 of 1) PTHR21281//PTHR21281:SF0 - UNCHARACTERIZED // CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTTGACGACCTGCTACCAAGAGTGAT |
| Internal bar code: | GAGTGTCGAAAGGCCAATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAATTGTCCTGCCACACA |
| Suggested primer 2: | GGTAAAGCTGAACGAGCAGG |