Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.150015 |
Chromosome: | chromosome_9 |
Location: | 659483 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g403200 | FAP198 | Flagellar Associated Protein | antisense | 5'UTR|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCAGTTGACGACCTGCTACCAAGAGTGAT |
Internal bar code: | GAGTGTCGAAAGGCCAATGTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 548 |
LEAP-Seq percent confirming: | 95.3846 |
LEAP-Seq n confirming: | 62 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCAATTGTCCTGCCACACA |
Suggested primer 2: | GGTAAAGCTGAACGAGCAGG |