Insertion junction: LMJ.RY0402.150015_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.150015
Chromosome:chromosome 13
Location:3742677
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACATGTGTGTGTGTGTGCTTTGCACCA
Internal bar code:GAATTCGGACGGGCGATCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:96.5583
LEAP-Seq n confirming:505
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACATTTGTTTCCACCTT
Suggested primer 2:TTCTTGAGGAATGCGGTCTT