Insertion junction: LMJ.RY0402.150036_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.150036
Chromosome:chromosome 17
Location:2457754
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g715801 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCCGGAGGTGGTGGAGTTCAACTTCAAC
Internal bar code:TTTCGGACGGCAAAAAGTAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:610
LEAP-Seq percent confirming:92.8053
LEAP-Seq n confirming:6269
LEAP-Seq n nonconfirming:486
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGCACCGTGGGATTCGTA
Suggested primer 2:CCTTCTTTCCTCCATCCCTC