| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150047 |
| Chromosome: | chromosome_12 |
| Location: | 4416937 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGACGCAAGGATGGGGCGGAGGAGGTACT |
| Internal bar code: | TCTGTAAAAACTGGGGCAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 99.893 |
| LEAP-Seq n confirming: | 1867 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCAGAAAGAGGACTTGA |
| Suggested primer 2: | TAGTCATAGTCCGCCTCGCT |