| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150075 |
| Chromosome: | chromosome 7 |
| Location: | 2352205 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328226 | (1 of 6) IPR000817 - Prion protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTACCTGTCTACTTCGAGACTCAGGCCG |
| Internal bar code: | TGCCTGCCTGGTTCAGTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 61.9494 |
| LEAP-Seq n confirming: | 1983 |
| LEAP-Seq n nonconfirming: | 1218 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAACAGCAGCAGTAGTA |
| Suggested primer 2: | TTTGGGCTGGTATCTTCACC |