| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150084 |
| Chromosome: | chromosome 9 |
| Location: | 5654054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401219 | (1 of 1) IPR000210//IPR011041//IPR011333 - BTB/POZ domain // Soluble quinoprotein glucose/sorbosone dehydrogenase // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATGCACTCGCCAACAAAGCTTTTTCC |
| Internal bar code: | CGATTCGATCGGGGACCCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.6149 |
| LEAP-Seq n confirming: | 4915 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACATGTGCCAAACCAAG |
| Suggested primer 2: | GCCTCATGTCAGACCTCGTT |