| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150086 |
| Chromosome: | chromosome 12 |
| Location: | 5435023 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACAACCCGTTCTTGCCGATTCTACCT |
| Internal bar code: | ATGACGGGTTACCCCGAGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTTCAGCTGGAACCGT |
| Suggested primer 2: | GAAAAGTGAGGGGGAGAAGC |