| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150086 |
| Chromosome: | chromosome 13 |
| Location: | 2266184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578850 | MOT27 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCCGGGTGGCTCGGGTGGCCTGGGTG |
| Internal bar code: | TTATCATGGCCACGTAAGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 91.6501 |
| LEAP-Seq n confirming: | 461 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCGCCCTACTGTACCC |
| Suggested primer 2: | CGAGACAGAGGTCAGAAGCC |