| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150089 |
| Chromosome: | chromosome 2 |
| Location: | 2459792 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091900 | FAP301 | Flagellar Associated Protein 301 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTGCACCACTACCACGGCGACCTGCG |
| Internal bar code: | TTGTTTTACAGTGTATAGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.6103 |
| LEAP-Seq n confirming: | 3834 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCAAGAAATCCCAATCA |
| Suggested primer 2: | ATTTTGCTGGGTGGAAAGTG |