| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150114 |
| Chromosome: | chromosome 12 |
| Location: | 6826646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561350 | (1 of 1) PTHR13244//PTHR13244:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCTATGGCAGCGAGGGGGGGGGCTTAC |
| Internal bar code: | GGGCATTAAAATTTCAGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 96.5994 |
| LEAP-Seq n confirming: | 909 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGACCATGAGGGACTTG |
| Suggested primer 2: | ATAGCGATAATGGTGCCCAG |