| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150117 |
| Chromosome: | chromosome 9 |
| Location: | 6990464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410800 | NRT2B,NAR4 | (1 of 4) K02575 - MFS transporter, NNP family, nitrate/nitrite transporter (NRT, narK, nrtP, nasA); High affinity nitrate transporter | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATGACAGCCTCGATCGCAAACGCTGCGT |
| Internal bar code: | GAATTGCAATGTTGTCGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.537 |
| LEAP-Seq n confirming: | 1505 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTTCAGCCTTTTTGAA |
| Suggested primer 2: | ATACGCGCACACACAACAAT |