| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150121 |
| Chromosome: | chromosome 12 |
| Location: | 9494258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540927 | (1 of 1) K10738 - DNA helicase MCM9 [EC:3.6.4.12] (MCM9) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGGAACGCCAGATTTGCACCACCACC |
| Internal bar code: | CGGAAGGCCGCGGGCCAACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 99.639 |
| LEAP-Seq n confirming: | 6625 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCAAAGCGTGACAAAA |
| Suggested primer 2: | TGAGTTTGTCAGGTTGCCAG |