Insertion junction: LMJ.RY0402.150123_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.150123
Chromosome:chromosome 6
Location:3487667
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278101 STD1 Serine/threonine dehydratase; (1 of 1) 4.3.1.17 - L-serine ammonia-lyase / Serine deaminase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACGCGACTGTTTCCTTACGGGATTGGC
Internal bar code:CTGTGTGGTGATGCGCGTCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTGTGTGTGTGTGTGT
Suggested primer 2:GAGAAAGGGGTGAGGGTTTC