| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150127 |
| Chromosome: | chromosome 1 |
| Location: | 1863129 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010150 | (1 of 3) IPR006073//IPR027417 - GTP binding domain // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTGCTGGGACTCCCGCGTGGGAGGCC |
| Internal bar code: | GCACGGCCGAGATATCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.4528 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATATAGCTCAGGAGGGC |
| Suggested primer 2: | CCTTGCCAGGGTTATGAAAA |