| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150131 |
| Chromosome: | chromosome 6 |
| Location: | 7844817 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302950 | (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGTCTGCGGCGTCGCGAAGGACTTG |
| Internal bar code: | GGAGAGAGCCGAGTATCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 96.9231 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGCGTAAGGATCTGGG |
| Suggested primer 2: | ACTTCCACTCGCACTCGG |