| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150137 |
| Chromosome: | chromosome 10 |
| Location: | 3064345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441500 | (1 of 1) K09602 - ubiquitin thioesterase protein OTUB1 [EC:3.4.19.12] (OTUB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTTAAGATGGGCATAAAGCATTAGCC |
| Internal bar code: | ATGGTTTAGCGGGATCGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 99.6516 |
| LEAP-Seq n confirming: | 572 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAGGAGCTTGTGCAGGT |
| Suggested primer 2: | TGGTGTACTTGGACAGCAGC |