Insertion junction: LMJ.RY0402.150144_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.150144
Chromosome:chromosome 17
Location:1258625
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g705350 CDPKK2 (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/Calmodulin Dependent Protein Kinase Kinase Homolog 2 isoform beta putative CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGATGACCTCCACCGCCACCACCTCCTGC
Internal bar code:CAACGTCAGACCACTAGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:91.8304
LEAP-Seq n confirming:2383
LEAP-Seq n nonconfirming:212
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAGTAACGCACAGCACG
Suggested primer 2:CCCTTTACACATCACACCCC