| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150170 |
| Chromosome: | chromosome_5 |
| Location: | 2128765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234640 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGATGCAAACACGAATATGGTTTTATGCC |
| Internal bar code: | CTTGTAGAGCTGCCCGTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCCAGTCCAACCTGAAC |
| Suggested primer 2: | CTCCTTGAACCCCTTGAACA |