| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150194 |
| Chromosome: | chromosome 7 |
| Location: | 6398366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357900 | (1 of 1) K08054 - calnexin (CANX) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGTTGAGGAAGCTGTCTGCCAGCGACT |
| Internal bar code: | GGACGAATGCTCAACGTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.3615 |
| LEAP-Seq n confirming: | 1245 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCGAGCACAAAAGCAGA |
| Suggested primer 2: | CACCCTCGCTTTCCATGTAT |