Insertion junction: LMJ.RY0402.150197_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.150197
Chromosome:chromosome 17
Location:6808295
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g744997 PNO4 FAD dependent oxidoreductase; (1 of 1) PTHR10617//PTHR10617:SF52 - ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE // OXIDOREDUCTASE/ELECTRON CARRIER-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAACTCCCCGCCCCCGCCTTTGCTCCTG
Internal bar code:AATTTCTACGCTGTCTGCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:36.4214
LEAP-Seq n confirming:401
LEAP-Seq n nonconfirming:700
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGTGAAACTAGCGGAGC
Suggested primer 2:TACCGCTTTTCCAAACATCC