| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150200 |
| Chromosome: | chromosome 2 |
| Location: | 776695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078777 | PHC16 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATTCGAAACTGTGGACCGTTGGTCCC |
| Internal bar code: | GGCCTCGGTACTCTCATTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 1080 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTACCCGCATGTACTCCT |
| Suggested primer 2: | AGGTATTGCCGTTCCAAGTG |