| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150213 |
| Chromosome: | chromosome 3 |
| Location: | 3872229 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171050 | GHL1 | (1 of 2) 3.2.1.21 - Beta-glucosidase / Gentobiase; Glycosyl hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCCGGTGTGGTGGGGCCTTGTCGGGC |
| Internal bar code: | ATCGTTCGTGGGATGTCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCTTGTGCTGTCCTTAC |
| Suggested primer 2: | TACTAGGTTAGGCGAGCGGA |