| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150227 |
| Chromosome: | chromosome 14 |
| Location: | 2294294 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623403 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGCGTGACAAGCAATTTCCTGCTCCA |
| Internal bar code: | TGTGCGTATTGTAATGCAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.5357 |
| LEAP-Seq n confirming: | 3430 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACACCGGCTCCCTGTA |
| Suggested primer 2: | TGTCTACTCTGCACATCGGC |