| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150266 |
| Chromosome: | chromosome 4 |
| Location: | 3946387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231222 | CPN60A,CPN60 alpha | Chaperonin 60A; (1 of 1) PTHR11353:SF11 - CHAPERONIN 60 SUBUNIT ALPHA 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGCAAAGGACCGCAAACAGCCGCGCAG |
| Internal bar code: | GTCGCCTTGCAAGAGTGTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 98.4906 |
| LEAP-Seq n confirming: | 522 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCCTGCCTACATTCCC |
| Suggested primer 2: | GTGCAGGTCTCCGACTCTTC |