| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150269 |
| Chromosome: | chromosome 4 |
| Location: | 1933325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218250 | FAP313,ARL3 | Flagellar Associated Protein 313; (1 of 1) K07944 - ADP-ribosylation factor-like protein 3 (ARL3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGGGCCGTCCAGCCGGCCGCGGTGCCG |
| Internal bar code: | GAGGCCGGGCGGTAAGCCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 99.7908 |
| LEAP-Seq n confirming: | 11449 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTTCGGGTGTAGGTGC |
| Suggested primer 2: | TGCTATCAGACATGGCTTGC |