Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.150269 |
Chromosome: | chromosome_4 |
Location: | 1933610 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g218250 | ARL3 | ARF-like GTPase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCTGCATCCCCCTTGCAGCCCCACGCCGC |
Internal bar code: | AATTGTTGATTAGCAATAGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 150 |
LEAP-Seq percent confirming: | 81.7343 |
LEAP-Seq n confirming: | 443 |
LEAP-Seq n nonconfirming: | 99 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTGGTGCATGGGTGTAAG |
Suggested primer 2: | TCAGGAAAGAATGGTGGGAG |