Insertion junction: LMJ.RY0402.150283_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.150283
Chromosome:chromosome 6
Location:1544043
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g260050 (1 of 27) IPR015947 - PUA-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGCCAGCGCGCCTCTAGTGAGCAACTT
Internal bar code:CTATTCGGTAAGTGGACACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCATTTCACAGAACACCC
Suggested primer 2:AAGCTATAGAAGCCACGCCA