| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150301 |
| Chromosome: | chromosome 16 |
| Location: | 4749003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686750 | PTA3 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTGCCCCTTCCTCACCTCATTGCGTGC |
| Internal bar code: | CAAAGCTTAGCTGTTGTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.8024 |
| LEAP-Seq n confirming: | 1010 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCATTGGTTTGGGGTCTA |
| Suggested primer 2: | AGCGTGTCCAAGCCTACAGT |