| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150305 |
| Chromosome: | chromosome 1 |
| Location: | 5029980 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034800 | (1 of 1) PTHR21477//PTHR21477:SF12 - FAMILY NOT NAMED // CF9 PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTCTGGGGGAGTCTGGGGAGTGGGGGC |
| Internal bar code: | TTGGGTAGGGCTAGTGATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 99.7221 |
| LEAP-Seq n confirming: | 5023 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCACCGCTAGAGTTACCG |
| Suggested primer 2: | TGTAAGGGAGGTGCCTGAAC |