| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150305 |
| Chromosome: | chromosome 6 |
| Location: | 3680672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278139 | RAB18B | Small Rab-related GTPase; (1 of 3) K07910 - Ras-related protein Rab-18 (RAB18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCCCCACCATTCCAGCCAGCGTGCCC |
| Internal bar code: | CCGGGCGGCAGCTGCTCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1110 |
| LEAP-Seq percent confirming: | 99.7263 |
| LEAP-Seq n confirming: | 2186 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACGTTCCTGAATTCCT |
| Suggested primer 2: | TGAGCGTTTGGCTTGTAGTG |