| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150308 |
| Chromosome: | chromosome 5 |
| Location: | 3427307 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241250 | (1 of 1) IPR000104//IPR000253//IPR001357 - Antifreeze protein, type I // Forkhead-associated (FHA) domain // BRCT domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTCCAAGGCCTCCTCCAGCTGCTGCTC |
| Internal bar code: | TTTTAGTGGTGCTTACCCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 99.617 |
| LEAP-Seq n confirming: | 4161 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGCTTCCATTTCTCCA |
| Suggested primer 2: | CTGCTCCTCTTCCTCCTCCT |