| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150312 |
| Chromosome: | chromosome 12 |
| Location: | 2077354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509500 | (1 of 1) IPR000595//IPR002048//IPR011992//IPR018490//IPR029530 - Cyclic nucleotide-binding domain // EF-hand domain // EF-hand domain pair // Cyclic nucleotide-binding-like // Cell division control protein 31 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATAAGCCGCGGCTGCCTTTTCCTCGA |
| Internal bar code: | GCTGCTTGAGCGCGCCTGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1094 |
| LEAP-Seq percent confirming: | 98.7044 |
| LEAP-Seq n confirming: | 2057 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCCGTCCTTACTTGTTG |
| Suggested primer 2: | GAAGTTGCGGTTTCTTCGAG |